![]() ![]() In general, the major role of DNA polymerases is to extend the primer strand by incorporating dNTP opposite the template base (or the lesion). Typhoon System (GE Healthcare Life Sciences) Sequi-Gen GT Nucleic Acid Sequencing Cell, 38 × 50 cm (Bio-Rad Laboratories) RQF-3 Rapid Quench-Flow Instrument (KinTek Corporation) ![]() Luer Lock disposable syringes, 1 mL and 5 mL (Bio-Rad Laboratories) ImageJ software (National Institutes of Health) Urea (electrophoresis grade, Sigma-Aldrich)ĭry block heater and thermometer (VWR International) N,N,N,N-Tetramethylethylenediamine (TEMED) (Bio-Rad Laboratories)ĥX TBE buffer (0.445M Tris-HCl, 0.445M boric acid, and 0.01 M EDTA) Human DNA polymerase η (hpol η) R61M (N-terminal 1–432 amino acids, expressed and purified as described ( Biertumpfel et al., 2010 Su et al., 2015) ![]() 40% Acrylamide/bis, 19:1, w/v, 5% crosslinker (Bio-Rad Laboratories)Īmmonium persulfate (APS) (Bio-Rad Laboratories)īovine serum albumin (BSA), 2 mg/mL (Pierce Protein Biology Products)ĭL-Dithiothreitol (DTT) (Research Products International)ĭNA primer: 5´-/FAM/CGG GCT CGT AAG CGT CAT-3´ (Integrated DNA Technologies)ĭNA template: 5´-TCA T( 8-oxodG)A TGA CGC TTA CGA GCC CG-3´ (Integrated DNA Technologies)
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